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Literature summary extracted from

  • Hellmuth, S.; Poehlmann, C.; Brown, A.; Boettger, F.; Sprinzl, M.; Stemmann, O.
    Positive and negative regulation of vertebrate separase by cdk1-cyclin b1 may explain why securin is dispensable (2015), J. Biol. Chem., 290, 8002-8010.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.49 expressed in HEK-293T cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.49 S1126A the mutation prevents the conformational change of the enzyme caused by phosphorylation, thereby rendering separase resistant to precipitation in mitosis Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.49 cyclin-dependent kinase 1-cyclin B1 Cdk1-cyclin B1 triggers precipitation of separase by phosphorylation but stabilizes it by inhibitory binding. Only separase that is first complexed by Cdk1-cyclin B1 can later be activated by cyclin B1 degradation Homo sapiens
3.4.22.49 securin
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.49 cohesin + H2O Homo sapiens
-
 ?
-
 ?
3.4.22.49 Scc1 + H2O Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.49 Homo sapiens Q14674
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.49 phosphoprotein mitosis-specific phosphorylation of Ser-1126 decreases the solubility of separase by cyclin-dependent kinase 1-cyclin B1 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.49 HCT-116 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.49 cohesin + H2O
-
 Homo sapiens ?
-
 ?
3.4.22.49 Scc1 + H2O
-
 Homo sapiens ?
-
 ?

General Information

EC Number General Information Comment Organism
3.4.22.49 physiological function the enzyme cleaves the Scc1/Rad21 subunit of cohesin, thereby triggering chromosome segregation Homo sapiens